| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Ashley O (10)
|
MAUN-3 |
28.04 |
27.71 |
-0.33 |
| 2 |
Waslyn, Rylee E (10)
|
MAWAC |
30.24 |
28.49 |
-1.75 |
| 3 |
Vergara, Viviana E (10)
|
MACBST |
29.72 |
28.53 |
-1.19 |
| 4 |
Evans, Olivia M (10)
|
MASEA |
28.83 |
28.64 |
-0.19 |
| 5 |
Feuzeu Mekoue, Laurielle Kel (10)
|
MASSC |
29.94 |
29.04 |
-0.90 |
| 6 |
Saul, Yuran A (10)
|
MAJRAY |
30.20 |
29.64 |
-0.56 |
| 7 |
Hahn, Lily (9)
|
MARAD |
31.10 |
29.72 |
-1.38 |
| 8 |
Cassidy, Megan E (10)
|
MAGRA |
29.76 |
29.85 |
0.09 |
| 9 |
Dimartile, Meghan (10)
|
MAUDAC |
29.93 |
29.89 |
-0.04 |
| 10 |
Szerencsits, Olivia S (10)
|
MAPAAC |
30.30 |
29.94 |
-0.36 |
| 11 |
Brandt, Mackenzie A (10)
|
MAWAC |
30.81 |
30.00 |
-0.81 |
| 12 |
Turco, Lily M (10)
|
MAGRA |
30.55 |
30.07 |
-0.48 |
| 13 |
Lunn, Emma K (9)
|
MASSC |
30.14 |
30.46 |
0.32 |
|
Wilson, Taylor L (10)
|
MARAD |
31.07 |
NS |
- |
|
Wells, De'jah (10)
|
MASSC |
30.84 |
NS |
- |